abstract：:One hundred eight consecutive patients with indolent lymphoproliferative diseases were stratified into chronic lymphocytic leukemia (CLL), stage III and IV well-differentiated lymphocytic lymphoma (WDLL), and stage III and IV follicular lymphoma (FL). Within each stratum, patients were prospectively and randomly assig...

journal_title：Blood

authors： Jacobs P,King HS

更新日期：1987-06-01 00:00:00

abstract：:A simple and reliable cytogenetic test for Fanconi's anemia (FA) that is based on the hypersensitivity of FA cells to mitomycin C (MC) is described. Equal volumes of whole blood from a patient in whom the diagnosis of FA is suspected and from a normal person of the opposite sex are co-cultured in phytohemagglutinin-co...

journal_title：Blood

authors： German J,Schonberg S,Caskie S,Warburton D,Falk C,Ray JH

更新日期：1987-06-01 00:00:00

abstract：:Interleukin-2 (IL-2) induces differential secretion of lymphokines by IL-2 receptor (IL-2R)-positive and IL-2R-negative T cells. We studied T cell IL-2R-specific modulation of adult bone marrow erythropoiesis by recombinant IL-2 (rIL-2). I3-2R were induced by CD3 T cell surface determinant-triggering and analyzed by c...

journal_title：Blood

authors： Burdach SE,Levitt LJ

更新日期：1987-05-01 00:00:00

abstract：:Adenosine deaminase (ADA) is an enzyme in the purine catabolic pathway that has been used as an enzymatic marker of T cell lymphoblastic malignancies due to its high specific activity in thymocytes and immature T cells. We have investigated whether the level of ADA activity in lymphoid leukemic cells correlates with t...

journal_title：Blood

authors： Gan TE,Dadonna PE,Mitchell BS

更新日期：1987-05-01 00:00:00

abstract：:Endothelial cell prostacyclin (PGI2) inhibits platelet activation by raising platelet cyclic AMP. Previously, platelet activation was also shown to be blocked by plasmin formed by endothelium-derived tissue plasminogen activator (TPA). We have now studied interactions between PGI2 and plasmin in the control of platele...

journal_title：Blood

authors： Schafer AI,Zavoico GB,Loscalzo J,Maas AK

更新日期：1987-05-01 00:00:00

abstract：:The genes for the hemopoietic growth factors, GM colony-stimulating factor (CSF) and G-CSF have been cloned, and recombinant material is available for both. We tested these recombinant factors for their effects on the blast cells of acute myeloblastic leukemia (AML). Culture methods are available that support both col...

journal_title：Blood

authors： Kelleher C,Miyauchi J,Wong G,Clark S,Minden MD,McCulloch EA

更新日期：1987-05-01 00:00:00

abstract：:The activities of four purified human growth factors: biosynthetic (recombinant) granulocyte-macrophage colony-stimulating factor (GM-CSF); recombinant erythroid-potentiating activity (EPA); natural and recombinant pluripoietin (Ppo); and natural pluripoietin alpha (Ppo alpha), were compared on the growth of hematopoi...

journal_title：Blood

authors： Strife A,Lambek C,Wisniewski D,Gulati S,Gasson JC,Golde DW,Welte K,Gabrilove JL,Clarkson B

更新日期：1987-05-01 00:00:00

abstract：:We used our recently reported stable, transformed human renal carcinoma cell line as a model system to study the role of 3',5'-adenosine monophosphate (cAMP) in erythropoietin secretion. The erythropoietin produced by these cells is both biologically active and immunologically cross-reactive with purified native human...

journal_title：Blood

authors： Sherwood JB,Burns ER,Shouval D

更新日期：1987-04-01 00:00:00

abstract：:Selective overexpression (50- to 100-fold) in adult erythroid cells of either G gamma or A gamma fetal globin gene is observed in hereditary conditions known as delta beta zero-thalassemia and hereditary persistence of fetal hemoglobin (HPFH). Recently, a C----T change at position -196 of an overexpressed A gamma glob...

journal_title：Blood

authors： Ottolenghi S,Giglioni B,Pulazzini A,Comi P,Camaschella C,Serra A,Guerrasio A,Saglio G

更新日期：1987-04-01 00:00:00

abstract：:Acquisition of mature T cell function and the T cell antigen receptor repertoire occur in the thymus. In an effort to delineate the cascade of events leading to T cell maturation, we analyzed a series of clonal human precursor T cell neoplasms representing early, middle, and late stages of intrathymic differentiation....

journal_title：Blood

authors： Pittaluga S,Uppenkamp M,Cossman J

更新日期：1987-04-01 00:00:00

abstract：:The presence of circulating tumor idiotype interferes with the in vivo effectiveness of anti-idiotype antibodies. We developed two assays that permit identification of patients with high levels of serum idiotype without the need for first producing an anti-idiotype antibody. A cell suspension made from the tumor was c...

journal_title：Blood

authors： Miller RA,Lowder JN,Gralow J,Meeker T,Levy R

更新日期：1987-04-01 00:00:00

abstract：:The effect of shear force (depending on shear rate and viscosity of extracellular medium) and hematocrit of RBC suspension on RBC deformation was studied quantitatively using a cone-plate rheoscope with various kinds of cells, ie, partially hemolyzed (PH) cells, density-fractionated intact cells, and diamide-treated c...

journal_title：Blood

authors： Kon K,Maeda N,Shiga T

更新日期：1987-03-01 00:00:00

abstract：:Human platelets contain tortuous channels in their cytoplasm, the surface-connected or open canalicular system (OCS), that communicate directly with the surrounding medium through openings on the surface membrane. Some workers have suggested that the OCS serves as the egress route for products secreted during the rele...

journal_title：Blood

authors： White JG

更新日期：1987-03-01 00:00:00

abstract：:We have shown that cytosol samples from human leukemia cells frequently contain glucocorticoid receptor fragments that have a mol wt (Mr) of approximately 52,000. In the present study we demonstrate that the Mr approximately 52,000-receptor fragments are derived from intact glucocorticoid receptors (Mr approximately 9...

journal_title：Blood

authors： Distelhorst CW,Miesfeld R

更新日期：1987-03-01 00:00:00

abstract：:Hematologic engraftment and immune reconstitution were examined in patients who received cyclophosphamide and total body irradiation therapy followed by infusion of autologous bone marrow purged with anti-B1 monoclonal antibody (MoAb) and complement as therapy for non-Hodgkin's lymphoma. Hematologic engraftment was pr...

journal_title：Blood

authors： Anderson KC,Ritz J,Takvorian T,Coral F,Daley H,Gorgone BC,Freedman AS,Canellos GP,Schlossman SF,Nadler LM

更新日期：1987-02-01 00:00:00

abstract：:The causes of anemia and immunosuppression, major outcomes of malaria, are not well established. This study was undertaken to investigate whether erythropoietin (EP) production is adequate and whether the hemopoietic stem cells (CFU-S) were affected during the course of infection. Groups of female Balb/c mice infected...

journal_title：Blood

authors： Silverman PH,Schooley JC,Mahlmann LJ

更新日期：1987-02-01 00:00:00

abstract：:The glycoprotein (GP) IIb-IIIa complex was isolated from human platelet membranes by immunoaffinity chromatography using a monoclonal antibody specific for GP IIb-IIIa. GP IIb and IIIa were further separated in the presence of sodium dodecyl sulfate (SDS) by gel filtration high-performance liquid chromatography (HPLC)...

journal_title：Blood

authors： Hiraiwa A,Matsukage A,Shiku H,Takahashi T,Naito K,Yamada K

更新日期：1987-02-01 00:00:00

abstract：:The formation and functioning of the factor X activating complex on the surface of cultured human venous endothelial cells (HVEC) were investigated. To the HVEC monolayer human factors IXa, VIII, X, CaCl2, and S-2222 were added, and a gradually increasing activation of factor X was observed. The maximum activity of 88...

journal_title：Blood

authors： Váradi K,Elödi S

更新日期：1987-02-01 00:00:00

abstract：:A male neonate with Down's syndrome and congenital myeloproliferative disorder was studied. His blood picture showed the unique coexistence of leukocytosis with matured cells and a large number of blast cells. The in vitro proliferation and differentiation of blast cells into various lineages in the presence of phytoh...

journal_title：Blood

authors： Suda J,Eguchi M,Akiyama Y,Iwama Y,Furukawa T,Sato Y,Miura Y,Suda T,Saito M

更新日期：1987-02-01 00:00:00

abstract：:During pregnancy the plasma concentration of two different inhibitors of plasminogen activators (PAIs) increases. The only one found in the plasma of nonpregnant women (PAI1) is immunologically related to a PAI of endothelial cells; its plasma activity, as deduced from the inhibition of single-chain tissue-type plasmi...

journal_title：Blood

authors： Kruithof EK,Tran-Thang C,Gudinchet A,Hauert J,Nicoloso G,Genton C,Welti H,Bachmann F

更新日期：1987-02-01 00:00:00

abstract：:Previous in vitro findings suggest a critical role for the polymorphonuclear leukocyte (PMN) membrane glycoprotein complex CD18 in PMN adherence and chemotaxis. We examined the effect of the murine monoclonal antibody (MoAb) 60.3, recognizing CD18, on induced PMN accumulation in vivo. Rabbits were pretreated with MoAb...

journal_title：Blood

authors： Arfors KE,Lundberg C,Lindbom L,Lundberg K,Beatty PG,Harlan JM

更新日期：1987-01-01 00:00:00

abstract：:A myeloma cell-reactive monoclonal antibody (MoAb), MM4, was generated from BALB/c mice immunized with alternate injections of cells from two human multiple myeloma (MM) cell lines. Screening by the enzyme-linked immunosorbent assay (ELISA) technique showed that MM4 reacted with human MM cell lines (7 of 7 positive), ...

journal_title：Blood

authors： Tong AW,Lee JC,Stone MJ

更新日期：1987-01-01 00:00:00

abstract：:Factor IXAlabama is a variant factor IX molecule responsible for a clinically moderate form of hemophilia B. Twenty-five kilobases (kb) of the variant gene, including seven exons coding for the structural protein, were cloned and characterized. The restriction map and the arrangement of coding regions are identical to...

journal_title：Blood

authors： Davis LM,McGraw RA,Ware JL,Roberts HR,Stafford DW

更新日期：1987-01-01 00:00:00

abstract：:Although a number of studies have indicated correlations between histologic subtypes of tumors and certain nonrandom chromosome changes, cytogenetic studies of lymphoma are in an early stage compared to those of leukemia. No comprehensive analysis of available data has so far been attempted in the literature either. H...

journal_title：Blood

authors： Koduru PR,Filippa DA,Richardson ME,Jhanwar SC,Chaganti SR,Koziner B,Clarkson BD,Lieberman PH,Chaganti RS

更新日期：1987-01-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay for free tissue-type plasminogen activator (t-PA) in human blood was developed based on a murine monoclonal antibody directed against the active site of t-PA. The lower limit of sensitivity of the assay applied to plasma is 2 ng/mL for one-chain t-PA but only 100 ng/mL for two-chai...

journal_title：Blood

authors： Holvoet P,Boes J,Collen D

更新日期：1987-01-01 00:00:00

abstract：:Stimulators of human erythroid burst-forming units (BFU-E) and multipotential colony-forming cells (CFU-GEMM) can be produced by a number of different cell types. A product of human peripheral blood monocytes, interleukin 1 (IL-1), was evaluated for its ability to stimulate fibroblast cultures to produce stimulators o...

journal_title：Blood

authors： Zucali JR,Broxmeyer HE,Dinarello CA,Gross MA,Weiner RS

更新日期：1987-01-01 00:00:00

abstract：:The present studies were initiated to define the coding region of a 34 kilodalton (kd) protein (p34) frequently observed with antibodies from HTLV-III/LAV-infected people by immunoblotting and radioimmunoprecipitation (RIP) techniques. We have directly mapped this viral protein to the pol gene of HTLV-III/LAV by radio...

journal_title：Blood

authors： Allan JS,Coligan JE,Lee TH,Barin F,Kanki PJ,M'Boup S,McLane MF,Groopman JE,Essex M

更新日期：1987-01-01 00:00:00

abstract：:Epinephrine causes platelet aggregation and secretion by interacting with alpha 2-adrenergic receptors on the platelet surface. Platelet aggregation requires the binding of fibrinogen to a specific receptor on the membrane glycoprotein IIb-IIIa complex. Although the IIb-IIIa complex is identifiable on the surface of r...

journal_title：Blood

authors： Shattil SJ,Motulsky HJ,Insel PA,Flaherty L,Brass LF

更新日期：1986-12-01 00:00:00

abstract：:Nomifensine (Merital, Alival; Hoechst, Frankfurt, FRG), an antidepressant drug, may cause immune hemolytic anemia (IHA) of the so-called immune complex type that is believed to occur by means of an innocent-bystander mechanism. In this report we describe findings that are not consistent with this mechanism in a patien...

journal_title：Blood

authors： Salama A,Mueller-Eckhardt C

更新日期：1986-12-01 00:00:00

abstract：:Patients with severe aplastic anemia were conditioned with cyclophosphamide (200 mg/kg) and given marrow grafts from HLA-identical family members. Among 233 patients transplanted, 225 survived greater than or equal to 14 days and could be evaluated for engraftment. Forty-four of the 225 rejected their graft; 33 of the...

journal_title：Blood

authors： Deeg HJ,Self S,Storb R,Doney K,Appelbaum FR,Witherspoon RP,Sullivan KM,Sheehan K,Sanders J,Mickelson E

更新日期：1986-12-01 00:00:00

abstract：:One hundred forty-two patients between the ages of 1 and 17 years who survived disease-free more than 1 year after marrow transplantation for hematologic malignancy had growth and development evaluations from one to 14 years posttransplant (median 4 years). Prior to transplant all children received multiagent chemothe...

journal_title：Blood

authors： Sanders JE,Pritchard S,Mahoney P,Amos D,Buckner CD,Witherspoon RP,Deeg HJ,Doney KC,Sullivan KM,Appelbaum FR

更新日期：1986-11-01 00:00:00

abstract：:Detachment of individual sickle erythrocytes from cultured endothelial cell monolayers has been evaluated by a fluid-shearing technique in an effort to quantitate adherence at shear forces that would be anticipated in the in vivo circulation. Nonirreversibly sickled cells (non-ISC) were more adherent at normal oxygen ...

journal_title：Blood

authors： Smith BD,La Celle PL

更新日期：1986-11-01 00:00:00

abstract：:Conditioned medium derived from peripheral mononuclear low-density cells stimulated with phytohemagglutinin (PHA) supports the growth of noncommitted hematopoietic progenitors from marrow and peripheral blood cells. These immature progenitors (CFU-GEMM) can be identified in culture as multilineage hematopoietic coloni...

journal_title：Blood

authors： Kanz L,Löhr GW,Fauser AA

更新日期：1986-11-01 00:00:00

abstract：:Low-dose interferon-alpha (IFN-alpha) therapy is consistently effective in the treatment of hairy cell leukemia (HCL). In two cases of resistance to IFN-alpha administration, we diagnosed variant HCL, a form of HCL with intermediate features between typical HCL and B cell prolymphocytic leukemia. We tried to distingui...

journal_title：Blood

authors： Lehn P,Sigaux F,Grausz D,Loiseau P,Castaigne S,Degos L,Flandrin G,Dautry F

更新日期：1986-10-01 00:00:00

abstract：:Inhibition of complement-mediated granulocyte aggregation has recently been proposed as a mechanism of action of high-dose corticosteroids in shock states. Such inhibition may be effected through alteration of receptor function. Methylprednisolone inhibits binding of the synthetic chemotaxin f-methionine-leucine-pheny...

journal_title：Blood

authors： Skubitz KM,Hammerschmidt DE

更新日期：1986-10-01 00:00:00

abstract：:We compared the ability of aspirin to suppress platelet aggregation and thromboxane synthesis in ten normal subjects and ten patients with diabetic angiopathy and high rate of entry of new platelets into the circulation. When single doses of 100 to 1,000 mg aspirin were ingested daily for 1 month, there were time gaps...

journal_title：Blood

authors： DiMinno G,Silver MJ,Cerbone AM,Murphy S

更新日期：1986-10-01 00:00:00

abstract：:A gamma-chain variant with a lower molecular weight than the normal gamma chain was detected in a new congenital abnormal fibrinogen with impaired polymerization of the fibrin monomer and with normal release of fibrinopeptides A and B in a 45-year-old male. Purified fibrinogen analyzed on SDS-polyacrylamide gel electr...

journal_title：Blood

authors： Yoshida N,Okuma M,Moroi M,Matsuda M

更新日期：1986-09-01 00:00:00

abstract：:Lymphoepithelioid lymphoma (Lennert's lymphoma) was first described as a special variant of Hodgkin's disease. This lesion is characterized by a high percentage of epithelioid and T cells and rarely contains the classical Hodgkin's/Reed-Sternberg cells. Cytogenetic abnormalities indicate that Lennert's lymphoma is of ...

journal_title：Blood

authors： Feller AC,Griesser GH,Mak TW,Lennert K

更新日期：1986-09-01 00:00:00

abstract：:In previous studies we have found that monocytes produce soluble factors that stimulate human umbilical vein endothelial cells to produce granulocyte-macrophage colony-stimulating activity (CSA), burst-promoting activity (BPA), and megakaryocyte colony-stimulating activity (Meg-CSA) as well as factors that stimulate T...

journal_title：Blood

authors： Broudy VC,Zuckerman KS,Jetmalani S,Fitchen JH,Bagby GC Jr

更新日期：1986-08-01 00:00:00

abstract：:The autosomal recessive transmission of Glanzmann's thrombasthenia (GT) and Bernard-Soulier syndrome (BSS), together with requests of families who already had children with these diseases, prompted us to investigate the feasibility of their antenatal diagnosis. The preliminary step leading to the early detection of GT...

journal_title：Blood

authors： Gruel Y,Boizard B,Daffos F,Forestier F,Caen J,Wautier JL

更新日期：1986-08-01 00:00:00